amv reverse transcriptase 5x reaction buffer (Promega)

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Promega amv reverse transcriptase 5x reaction buffer

Amv Reverse Transcriptase 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amv+reverse+transcriptase+buffer/amv+reverse+transcriptase+buffer/pmc11834103-25-0-7
Average 90 stars, based on 1 article reviews

amv reverse transcriptase 5x reaction buffer - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress"

Article Title: TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress

Journal: Cell reports

doi: 10.1016/j.celrep.2024.115167

Figure Legend Snippet:

Techniques Used: Recombinant, Membrane, Reverse Transcription, Protease Inhibitor, Magnetic Beads, Staining, Mutagenesis, Silver Staining, BIA-KA, RNA Sequencing, Plasmid Preparation, Software

Incubation:

Article Title: Gut flora-derived extracellular vesicles, and method for searching for a disease model, vaccine, and candidate drug and for diagnosis using the same
Article Snippet: .. To each of the vesicles was added 2 μl of random decamer (Ambion, 5722G), followed by incubation at 95° C. for 10 min and at 75° C. for 10 and storage at 4° C. Each sample was treated with random decamer and incubated with AMV reverse transcriptase (Promega, M510F) 3 μl, AMV reverse transcriptase buffer (Promega, M515A) 4 μl, 10 mM dNTP 2 μl, and RNase inhibitor (Promega, N211B) 1 μl, for 10 min at 25° C. and then for 2 hours at 37° C. Of 20 μl of each sample treated with AMV reverse transcriptase, 1 μl was used to detect a 16S rRNA gene by PCR. .. For use in PCR, 1 μl of the sample was mixed with 0.5 μl of Taq enzyme (NEB, M0273S), 2 μl of Taq enzyme buffer (NEB, B9014S), 1 μl of 10 mM dNTP, 1 μl of 10 pM bacterial universal forward primer (5′aaggcgacgatccctagctg-3′; SEQ ID NO. 4), 1 μl of 10 pM bacterial universal reverse primer (5′ttgagcccggggatttcaca-3′; SEQ ID NO. 5), and 13.5 μl of deionized water.

Article Title: HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects.
Article Snippet: .. To generate cDNA, 1 mg of the respective RNA was incubated with 1 mM oligo-dT for 10 min at 658C, followed by reverse transcription using 0.5 mM deoxyribonucleotide triphosphates (dNTPs; Invitrogen), 20 U rRNasin (Promega, Madison, WI, USA) and 10 U AMV reverse transcriptase enzyme (Promega, Madison, WI, USA) in AMV reverse transcriptase buffer (Promega, Madison, WI, USA) for 1 h at 428C. cDNAs were then used in PCR reactions using specific forward and reverse primers for: GAPDH: (F) 5′-ACCACAGTCCATGCCATCAC-3′ (R) 5′-TCCACCACCCTGTTGCTGTA-3′ b-actin: (F) 5′-TCTTGGGTATGGAATCCTGTGGCA-3′ (R) 5′-ACTCCTGCTTGCTGATCCACATCT-3′ g-actin: (F) 5′-ACAGCTTTACCACCACTGCTGAGA-3′ (R) 5′-ACTCCTGCTTGCTGATCCACATCT-3′ p21: (F) 5′-CTCTTCTGCTGTGGGTCAGGA-3′ (R) 5′-GTGGGGCAAGTGCCTAGATA-3′ GAP43: (F) 5′-TTTGTTTCTTGGTGTTGTTATGGC-3′ (R) 5′-GAACGGAACATTGCACACACA-3′ Nova-1: (F) 5′-TGAGTGTCCCCATTATACGTCAG-3′ (R) 5′-TAAACAAGCATATCATTCCCTTTG-3′ Tau: (F) 5′-GGTCGAAGATTGGCTCTACTG-3′ (R) 5′-GCCAAGGAAGCAGACACTTC-3′ HuD: (F) 5′-ACGCATCCTGGTTGATCAAG-3′ (R) 5′-AGGACACTCTCATCAGAATC-3′ PCR products were electrophoresed on 2.0% agarose gels and visualized by ethidium bromide staining. .. Results were captured on a Kodak Gel Logic 200 gel documentation setup and quantified by using the accompanying Kodak 1D analysis software.

Article Title: Gut flora-derived extracellular vesicles, and method for searching for a disease model, vaccine, and candidate drug and for diagnosis using the same
Article Snippet: .. To each of the vesicles was added 2 μl of random decamer (Ambion, 5722G), followed by incubation at 95° C. for 10 min and at 75° C. for 10 and storage at 4° C. Each sample was treated with random decamer and incubated with AMV reverse transcriptase (Promega, M510F) 3 μl, AMV reverse transcriptase buffer (Promega, M515A) 4 μl, 10 mM dNTP 2 μl, and RNase inhibitor (Promega, N211B) 1 μl for 10 min at 25° C. and then for 2 hours at 37° C. Of 20 μl of each sample treated with AMV reverse transcriptase, 1 μl was used to detect a 16S rRNA gene by PCR. .. For use in PCR, 1 μl of the sample was mixed with 0.5 μl of Taq enzyme (NEB, MO273S), 2 μl of Taq enzyme buffer (NEB, B9014S), 1 μl of 10 mM dNTP, 1 μl of 10 μM bacterial universal forward primer (5′aaggcgacgatccctagctg-3′; SEQ ID NO: 4, 1 μl of 10 μM bacterial universal reverse primer (5′ttgagcccggggatttcaca-3′; SEQ ID NO: 5), and 13.5 μl of deionized water.

Reverse Transcription:

Article Title: In situ reverse transcription: the magic of strength and anonymity
Article Snippet: .. The following components were used in the reaction mixture (RM) during testing: 1 × AMV reverse transcriptase buffer (Promega, 5 × AMV reverse transcriptase buffer: 250 mM Tris–HCl, 250 mM KCl, 50 mM MgCl 2 , 2.5 mM spermidine, 50 mM DTT), 0.2 U/μl AMV reverse transcriptase (Promega), 0.4 U/μl RNasin (Promega), 0.25 mM dATP, dGTP, dCTP and dTTP (Promega), 0.25 mM 5-bromo-2′-deoxyuridine triphosphate (BrdUTP, Sigma Aldrich), 0.05 mM biotin-16-2′-deoxyuridine-5′-triphosphate (biotin-dUTP, Roche), 0.05 mM digoxigenin-11-2′-deoxyuridine-5′-triphosphate (digoxigenin-dUTP, Roche), 0.05 mM Alexa Fluor® 555-aha-2′-deoxyuridine-5′-triphosphate (alexa-dUTP, Invitrogen), 0.05 mM ChromaTide® fluorescein-12-2′-deoxyuridine-5′-triphosphate (fluorescein-dUTP, Invitrogen) and 0.01 μg/μl oligonucleotides. .. The following oligonucleotides were used: oligonucleotides consisting of 15, 20 or 25 deoxythymidines (oligo dT15, Promega; oligo dT20, oligo dT25, Generi Biotech), oligonucleotides consisting of 15 deoxyadenines (oligo dA15, Generi Biotech) or a random hexanucleotide (Promega).

Article Title: Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli.
Article Snippet: We studied the inflammatory and immune responses of bovine mammary epithelial cells (bMEC) infected by mastitis isolates of Staphylococcus aureus.. Primary cultures of bMEC were co-incubated separately with three strains of S. aureus and one strain of Escherichia coli.. Transcriptional levels and/or protein release of interleukin-8 (IL-8), growth related oncogene alpha (GRO-a), growth related oncogene beta (GRO-b), tumor necrosis factor alpha (TNF-a), interleukin-1b (IL-1b), transforming growth factor b1 (TGF-b1) and interleukin-10 (IL-10) were measured at 3, 10 and 24 h post-infection (PI).

Article Title: Staphylococcal-associated molecular patterns enhance expression of immune defense genes induced by IL-17 in mammary epithelial cells.
Article Snippet: Interleukin-17A (IL-17A) and IL-17F have been shown to mediate a crucial crosstalk between the immune system and various epithelial tissues, stimulating various defensive mechanisms to bacterial infections.. A number of studies have characterized the response to IL-17A and IL-17F of epithelial cells from airways, intestine, and skin, but not from the mammary gland.. To evaluate the potential contribution of IL-17 to the immune defense of the mammary gland, we analyzed the effects of recombinant bovine IL-17A and IL-17F on primary bovine mammary epithelial cells (MEC) by quantitative PCR and ELISA.

Article Title: Spliced Leader Trapping Reveals Widespread Alternative Splicing Patterns in the Highly Dynamic Transcriptome of Trypanosoma brucei
Article Snippet: .. 3 μg total RNA was used as a template for reverse transcription in 50 μl AMV reverse transcriptase buffer (Promega, USA) in the presence of 1mM dNTPs, 360 ng random hexamers, 80 U RNasIn (Promega, USA) and 60 U AMV reverse transcriptase (Promega, USA). ..

Article Title: Technical note: Validation of candidate reference genes for normalization of quantitative PCR in bovine mammary epithelial cells responding to inflammatory stimuli.
Article Snippet: Total RNA (1 μg) was then reverse transcribed to cDNA: 1 μg of RNA was incubated with 1 μg of random primers (Promega, Madison, WI) for 10 min at 65°C and then for 5 min on ice, in a final volume of 10 μL. .. Reverse transcription was carried out by adding AMV reverse transcriptase buffer, 4 mM of dNTP, 15 U of AMV reverse transcriptase, and 40 U of RNAsin (all reagents from Promega) in a final reaction volume of 30 μL. ..

Article Title: Gut flora-derived extracellular vesicles, and method for searching for a disease model, vaccine, and candidate drug and for diagnosis using the same
Article Snippet: .. To each of the vesicles was added 2 μl of random decamer (Ambion, 5722G), followed by incubation at 95° C. for 10 min and at 75° C. for 10 and storage at 4° C. Each sample was treated with random decamer and incubated with AMV reverse transcriptase (Promega, M510F) 3 μl, AMV reverse transcriptase buffer (Promega, M515A) 4 μl, 10 mM dNTP 2 μl, and RNase inhibitor (Promega, N211B) 1 μl for 10 min at 25° C. and then for 2 hours at 37° C. Of 20 μl of each sample treated with AMV reverse transcriptase, 1 μl was used to detect a 16S rRNA gene by PCR. .. For use in PCR, 1 μl of the sample was mixed with 0.5 μl of Taq enzyme (NEB, MO273S), 2 μl of Taq enzyme buffer (NEB, B9014S), 1 μl of 10 mM dNTP, 1 μl of 10 μM bacterial universal forward primer (5′aaggcgacgatccctagctg-3′; SEQ ID NO: 4, 1 μl of 10 μM bacterial universal reverse primer (5′ttgagcccggggatttcaca-3′; SEQ ID NO: 5), and 13.5 μl of deionized water.

Polymerase Chain Reaction:

Article Title: Gut flora-derived extracellular vesicles, and method for searching for a disease model, vaccine, and candidate drug and for diagnosis using the same
Article Snippet: .. To each of the vesicles was added 2 μl of random decamer (Ambion, 5722G), followed by incubation at 95° C. for 10 min and at 75° C. for 10 and storage at 4° C. Each sample was treated with random decamer and incubated with AMV reverse transcriptase (Promega, M510F) 3 μl, AMV reverse transcriptase buffer (Promega, M515A) 4 μl, 10 mM dNTP 2 μl, and RNase inhibitor (Promega, N211B) 1 μl for 10 min at 25° C. and then for 2 hours at 37° C. Of 20 μl of each sample treated with AMV reverse transcriptase, 1 μl was used to detect a 16S rRNA gene by PCR. .. For use in PCR, 1 μl of the sample was mixed with 0.5 μl of Taq enzyme (NEB, MO273S), 2 μl of Taq enzyme buffer (NEB, B9014S), 1 μl of 10 mM dNTP, 1 μl of 10 μM bacterial universal forward primer (5′aaggcgacgatccctagctg-3′; SEQ ID NO: 4, 1 μl of 10 μM bacterial universal reverse primer (5′ttgagcccggggatttcaca-3′; SEQ ID NO: 5), and 13.5 μl of deionized water.

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amv reverse transcriptase 5x reaction buffer (Promega)

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1) Product Images from "TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress"

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